|
KMID : 1007519960050010030
|
|
Food Science and Biotechnology
1996 Volume.5 No. 1 p.30 ~ p.33
|
|
|
The Most Specific Primers for the Identification of Listeria Monocytogenes by the Polymerase Chain Reaction Method
|
|
Ryu, Chung Ho
Cho, Sung Hwan/Lnoue, Satoshi/Igimi, Shizunobu/Kumagai, Susumu
|
|
|
Abstract
|
|
|
|
The most specific primers for indentification of Listeria monocytogenes by the polymerase chain reaction method (PCR) were obtained. In many cases, non-specific bands were observed on the agarose gel after electrophoresis of the PCR products. The PCR product using our original primers C and D was a single 161bp fragment, only in the case of L. monocytogenes. Other Listeria spp. and 14 bacteria were amplified no fragments.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|